Main Research topics
Department of Sport medicine, Third Faculty of Medicine, Charles University in Prague aims to elucidate processes that underlie, or mediate, the beneficial metabolic effects of lifestyle interventions on obesity-related metabolic disturbances. Our attention is paid to metabolic, endocrine and immune characteristics of adipose tissue (AT). We closely collaborate with French laboratory of Obesity research of INSERM in Toulouse and, also, we were included in the frame of several European projects supported by European Commission.
In our first studies, which were dedicated to lipolysis in AT during diet or physical activity intervention, we showed that weight– reducing hypocaloric diet increases basal and catecholamine- stimulated lipolysis in AT and that this diet-induced effect is genotype-dependent (Stich, Harant et al. 1997). In a search for mechanisms of this diet-induced lipolysis enhancement we revealed that, in obese women, the hypocaloric diet is associated with a suppression of α2 adrenoreceptor-mediated antilipolytic effect, (Stich, Marion-Latard et al. 2002, Koppo, Siklova-Vitkova et al. 2012) that is in obesity increased (Stich, De Glisezinski et al. 2000). Similar adaptations of AT were found in response to interventions with regular physical activity (De Glisezinski, Crampes et al. 1998, Stich, de Glisezinski et al. 1999). Moreover in the above mentioned studies we were among first teams that demonstrated the use of microdialysis of adipose tissue in in vivo studies in obese subjects. During the next period our attention was paid to the diet- and exercise-induced modifications of modelling and endocrine function of AT. In physical training studies we did not find training-induced changes in expression of adipokines produced in adipose tissue (Klimcakova, Polak et al. 2006, Polak, Klimcakova et al. 2006). In dietary studies, we demonstrated that macrophages and adipocytes in AT exhibit differential response to long term hypocaloric diet: while adipocytes and adipocyte-derived secretory products are suppressed during initial phase of the diet and return to baseline at weight maintenance state, the macrophages show opposite pattern of the response (Capel, Klimcakova et al. 2009, Siklova-Vitkova, Klimcakova et al. 2012). Interestingly, the same “opposite” behaviour of these the two cellular populations were demonstrated when adipocyte and macrophage expressions are related to obesity and metabolic status (Klimcakova, Roussel et al. 2011, Klimcakova, Roussel et al. 2011). Using a unique approach, i.e. flow cytometry analysis of the small adipose tissue samples derived from needle biopsies, we demonstrated reduction of macrophage infiltration in AT during the hypocaloric diet (Kovacikova, Sengenes et al. 2011). As metabolic status of an individual is related to the adipogenic capacity of AT, we studied effects of hypocaloric diet on the differentiation and other characteristics of adipocyte precursor cells (preadipocytes) and demonstrated an increased differentiation capacity of preadipocytes in response to weight loss (Rossmeislova, Malisova et al. 2013). Our latest projects are focused on the effect of i) exercise and supplementation of the diet with omega 3 fatty acids on adipose tissue in elderly, ii) lymphstasis on adipose tissue expansion, iii) intermittent hypoxia on systemic and adipose tissue metabolism.
Current researchers: Assoc. Prof. Vladimír Štich, PhD, Assoc. Prof. Jan Polák, PhD, Lenka Rossmeislová, PhD, Michaela Šiklová, PhD, Michal Koc, PhD
PhD students: Jana Kračmerová, MSc, Veronika Mayerová, MSc, Eva Krauzová, MD, Martin Weistenstein, MSc.
- PhD program in Biomedicine Research (Third Faculty of Medicine)
- PhD supervisors in specialization:
- Molecular biology, genetics and virology,
- Preventive Medicine
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